Differences in gene expression related to the results of obesity treatment, peak oxygen uptake and fatty acid metabolism measured in a cardiopulmonary exercise test.

Gruchała-Niedoszytko M; van der Vlies P; Niedoszytko P; Sanjabi B; Niedoszytko M; Kaczkan M; Pieszko M; Gierat-Haponiuk K; Śliwińska A; Szalewska D; Małgorzewicz S,

Polish Archives Of Internal Medicine [Pol Arch Intern Med] 2018 Mar 26.

INTRODUCTION The impact of obesity requires methods used on a large scale as cardiopulmonary exercise test (CPET). The gene expression may explain CPET results on molecular level.

OBJECTIVES The aim of the study was to compare gene expression in obesity, differences related to the CPET results.

PATIENTS AND METHODS The study group consisted of 9 obese and 7 controls. The treatment consisted of diet, rehabilitation, behavioural therapy. Diet was based on the body composition analysed by bioelectrical impedance (BIA), resting metabolic rate, and subjective patients’ preferences. The rehabilitation was tailored according to the CPET results: VO2peak, FAT g/h. Behavioural intervention focused on the diagnosis of health problems leading to obesity, lifestyle modification, training in self-assessment and development of healthy habits. The intensive treatment lasted for 12 weeks and consisted of consultations with a physician, dietitian and medical rehabilitation specialist. RNA was isolated from the whole blood. The analysis was performed on 47,323 transcripts, among which the high quality of the RNA was confirmed in the group of 32,379 entities. Illumina TotalPrep 96 RNA Amplification Kit was used. RESULTS The results of our study show differences in gene expression related to the CPET results indicating abnormalities in fat oxidation and maximal oxygen uptake. The genes with the crucial differences in expression were CLEC 12A, HLA DRB1, HLA DRB4, HLA-A29.1, IFIT1 LOC100133662.

CONCLUSIONS The differences in expression may be responsible for the effects of the treatment related to inflammation caused by obesity, which influences muscles, fat tissue and metabolism of fatty acids.